Whole Genome Sequencing (WGS)
We are currently accepting bacteria and eukaryotes with small genome sizes. Maximum data throughput for one run is 12-15 Gb. Users will get 150X coverage for 16 small whole genomes (< 5 Mb) using the 2x300 V3 sequencing pipeline. We also help users to prepare high molecular genomic DNA with additional costs. Please submit a request to get a quote and it's highly recommended to discuss with the DIVA team in advance.
Turnaround time
As low as 7 business days (depending on sample numbers and queue status).
PCR-free WGS
Required DNA amount: >200 ng of HMW DNA
User Guidance
Elute DNA in nuclease free water after DNA extraction.
Run a gel to verify the quality of your samples.
Use Nanodrop to verify DNA purity (260/280 ~1.8 and 260/230 ~ 2.0 -2.2) and Qubit to measure DNA concentration.
Upload gel images, a data sheet that contains sample names, sample concentrations, species and genome size to the request form. The DIVA team will work with you to evaluate your samples.
Put your samples in a box, label your name and date on the box and deliver 400 ng of DNA for each genome to FRZ-0307 4D, in 4203 (across from the Robotics lab, near the warm rooms).
Low Input WGS
Required DNA amount: >20 ng of HMW DNA
User Guidance
Elute DNA in nuclease free water after DNA extraction.
Use Nanodrop to verify DNA purity (260/280 ~1.8 and 260/230 ~ 2.0 -2.2) and Qubit to measure DNA concentration.
Run ZAG or High Sensitivity DNA kit on Bioanalyzer to evaluate if there's degradation in your samples.
Upload files of fragment analysis report, a data sheet that contains sample names, sample concentrations, species and genome size to the request form. The DIVA team will work with you to evaluate your samples.
Put your samples in a box, label your name and date on the box and deliver 50 ng of DNA for each genome to FRZ-0307 4D, in 4203 (across from the Robotics lab, near the warm rooms).
*This method requires PCR amplification and may introduce bias to your results.
